Recombinant CD147 anticorps

N° du produit ABIN7126987

L’anticorps Lapin Monoclonal anti-CD147 a été validé pour WB, FACS, IF, IHC (f) et AGG. Il convient pour détecter CD147 dans des échantillons de Humain.

Aperçu rapide pour Recombinant CD147 anticorps (ABIN7126987)

Antigène: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CD147 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Aggregation (AGG)

Détail du produit anti-CD147 anticorps

Specificité: This MAb recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human BSG protein

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Inhibits Homotypic Aggregation, Adhesion to Matrix Proteins, & Migation through Matrigel (Order w/o BSA / azide);,Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HSB2 cells. Human renal cell carcinoma, ovarian carcinoma or melanoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CD147

Antigène: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Autre désignation: BSG

Sujet: 5A11 Antigen, 5F7, Basigin, Blood brain barrier HT7 antigen BSG, Extracellular Matrix Metalloproteinase Inducer (EMMPRIN), Leukocyte Activation Antigen M6, Neurothelin, OK Blood Group Antigen, TCSF, Tumor Cell-derived Collagenase Stimulatory Factor,CD147 / EMMPRIN / Neurothelin
Cellular localisation: Cell surface

Poids moléculaire: 35kDa (non-reduced), 40kDa (reduced)

ID gène: 682, 501293

UniProt: P35613

Pathways: S100 Proteins